Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3
Identifieur interne : 000873 ( Main/Exploration ); précédent : 000872; suivant : 000874Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3
Auteurs : Shenglong Ling [République populaire de Chine] ; Chengwei Zhang [République populaire de Chine] ; Wei Wang [République populaire de Chine] ; Xiaoying Cai [République populaire de Chine] ; Lu Yu [République populaire de Chine] ; Fangming Wu [République populaire de Chine] ; Longhua Zhang [République populaire de Chine] ; Changlin Tian [République populaire de Chine]Source :
- Scientific Reports [ 2045-2322 ] ; 2016.
Abstract
Interferon-inducible transmembrane protein IFITM3 was known to restrict the entry of a wide spectrum of viruses to the cytosol of the host. The mechanism used by the protein to restrict viral entry is unclear given the unavailability of the membrane topology and structures of the IFITM family proteins. Systematic site-directed spin labeling (SDSL) and electron paramagnetic resonance (EPR) studies of IFITM3 in detergent micelles identified a single, long transmembrane helix in the C-terminus and an intramembrane segment in the N-terminal hydrophobic region. Solution NMR studies of the same sample verified the secondary structure distribution and demonstrated two rigid regions interacting with the micellar surface. The resulting membrane topology of IFITM3 supports the mechanism of an enhanced restricted membrane hemi-fusion.
Url:
DOI: 10.1038/srep24029
PubMed: 27046158
PubMed Central: 4820770
Affiliations:
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R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Chengwei" sort="Zhang, Chengwei" uniqKey="Zhang C" first="Chengwei" last="Zhang">Chengwei Zhang</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Hefei National Laboratory of Microscale Physical Sciences, School of Life Science, University of Science and Technology of China</institution>, Hefei, 230027,<country>P. R. 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China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Cai, Xiaoying" sort="Cai, Xiaoying" uniqKey="Cai X" first="Xiaoying" last="Cai">Xiaoying Cai</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Hefei National Laboratory of Microscale Physical Sciences, School of Life Science, University of Science and Technology of China</institution>, Hefei, 230027,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yu, Lu" sort="Yu, Lu" uniqKey="Yu L" first="Lu" last="Yu">Lu Yu</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>High Magnetic Field Laboratory, Chinese Academy of Sciences</institution>, Hefei, 230031,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Fangming" sort="Wu, Fangming" uniqKey="Wu F" first="Fangming" last="Wu">Fangming Wu</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>High Magnetic Field Laboratory, Chinese Academy of Sciences</institution>, Hefei, 230031,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Longhua" sort="Zhang, Longhua" uniqKey="Zhang L" first="Longhua" last="Zhang">Longhua Zhang</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Hefei National Laboratory of Microscale Physical Sciences, School of Life Science, University of Science and Technology of China</institution>, Hefei, 230027,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Tian, Changlin" sort="Tian, Changlin" uniqKey="Tian C" first="Changlin" last="Tian">Changlin Tian</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Hefei National Laboratory of Microscale Physical Sciences, School of Life Science, University of Science and Technology of China</institution>, Hefei, 230027,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a2"><institution>High Magnetic Field Laboratory, Chinese Academy of Sciences</institution>, Hefei, 230031,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Interferon-inducible transmembrane protein IFITM3 was known to restrict the entry of a wide spectrum of viruses to the cytosol of the host. The mechanism used by the protein to restrict viral entry is unclear given the unavailability of the membrane topology and structures of the IFITM family proteins. Systematic site-directed spin labeling (SDSL) and electron paramagnetic resonance (EPR) studies of IFITM3 in detergent micelles identified a single, long transmembrane helix in the C-terminus and an intramembrane segment in the N-terminal hydrophobic region. Solution NMR studies of the same sample verified the secondary structure distribution and demonstrated two rigid regions interacting with the micellar surface. The resulting membrane topology of IFITM3 supports the mechanism of an enhanced restricted membrane hemi-fusion.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Friedman, R L" uniqKey="Friedman R">R. L. Friedman</name></author><author><name sortKey="Manly, S P" uniqKey="Manly S">S. P. Manly</name></author><author><name sortKey="Mcmahon, M" uniqKey="Mcmahon M">M. McMahon</name></author><author><name sortKey="Kerr, I M" uniqKey="Kerr I">I. M. Kerr</name></author><author><name sortKey="Stark, G R" uniqKey="Stark G">G. R. 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John</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ling, Shenglong" sort="Ling, Shenglong" uniqKey="Ling S" first="Shenglong" last="Ling">Shenglong Ling</name></noRegion><name sortKey="Cai, Xiaoying" sort="Cai, Xiaoying" uniqKey="Cai X" first="Xiaoying" last="Cai">Xiaoying Cai</name><name sortKey="Tian, Changlin" sort="Tian, Changlin" uniqKey="Tian C" first="Changlin" last="Tian">Changlin Tian</name><name sortKey="Tian, Changlin" sort="Tian, Changlin" uniqKey="Tian C" first="Changlin" last="Tian">Changlin Tian</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Wu, Fangming" sort="Wu, Fangming" uniqKey="Wu F" first="Fangming" last="Wu">Fangming Wu</name><name sortKey="Yu, Lu" sort="Yu, Lu" uniqKey="Yu L" first="Lu" last="Yu">Lu Yu</name><name sortKey="Zhang, Chengwei" sort="Zhang, Chengwei" uniqKey="Zhang C" first="Chengwei" last="Zhang">Chengwei Zhang</name><name sortKey="Zhang, Longhua" sort="Zhang, Longhua" uniqKey="Zhang L" first="Longhua" last="Zhang">Longhua Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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